The Shyam Lab, led by Dr. Raji Shyam, is based at Indiana University School of Optometry. Since 2022, the team has used a DeNovix CellDrop FL Automated Cell Counter and a DS-11 Spectrophotometer in their research on the corneal endothelium.
Read the research spotlight below for a look at Dr. Shyam and her team’s work!
“We study cornea, the clear front window of the eye. A single layer of non-proliferative cells lining the back of the cornea, the endothelium, is responsible for corneal transparency. In corneal endothelial dystrophies, the endothelial cell death results in corneal opacity, and blindness. There is no cure for these conditions, the common treatment is corneal transplantation. In the Shyam Lab, we study the mechanisms that regulate endothelial functions, with the goal of devising therapies to treat corneal dystrophies.”
“CellDrop is crucial to our work with primary corneal endothelial cells. These cells are precious, and few in numbers. By obtaining accurate cell counts, we are able to optimize the cell numbers needed for various biochemical assays. We use the [DS-11] Spectrophotometer to measure protein, DNA and RNA concentrations. It is very sensitive, which is especially important since we handle very small sample volumes.”
“Both instruments are very easy to use, and training new lab members to use these has been a breeze!”
Featured publication:
Characterization of a Novel Mouse Model for Fuchs Endothelial Corneal Dystrophy
