Isolating nuclei is critical for single nuclear workflows such as RNA-seq and ATAC-seq. Ensuring that nonclustered, debris-free samples are obtained is crucial to successful library preparation. Even when debris does not affect library preparation itself, it is important to count nuclei accurately for single-cell analysis.
The resources in this eBook will give you a detailed look at our recommended solution for the automated counting of isolated nuclei, the DeNovix CellDrop™ Automated Cell Counter. We’ll explore how the CellDrop’s DirectPipette™ technology, dedicated nuclei counting software applications, and fluorescence capabilities combine to provide accurate nuclei count data.
What You’ll Learn
- Best practices for nuclei extraction and counting
- Choosing between fluorescence (Acridine Orange and Propidium Iodide) and Trypan Blue methods for nuclei counting
- How to QC nuclei samples prior to single-cell sequencing
- How to automate cell and nuclei counting for next generation sequencing
CellDrop™ Cell Counters
DirectPipette™ technology eliminates the need for slides and eliminates plastic waste from cell counting workflows. Available with Dual Fluorescence and / or Brightfield.
