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Automated Hepatocyte Counting

Automated Hepatocyte Counting

Using machine learning, DeNovix scientists developed a unique algorithm for the CellDrop Automated Cell Counter to deliver rapid and reliable hepatocyte counts. Learn more about the Hepatocytes app in this collection of resources.

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CellDrop Automated Cell Counter

Hepatocyte Counting and Viability

Traditional automated cell counters have failed to reliably count hepatocytes due to irregular shapes, variable nuclei number, autofluoresence and often a high amount of debris in samples. DeNovix cell biologists have used advanced machine learning tools to develop the first robust and reliable method for automating the counting of these complex samples.

Quick and Simple

A mix-and-measure assay combined with dual fluorescence measurement and analysis in as little as 40 seconds

Accurate Analysis

Delivers data equivalent to an experienced technician, improving reproducibility and standardizing measurement

Comprehensive Data Analysis

In addition to live / dead hepatocytes, this application also reports the number of lymphocytes, free nuclei and debris objects
Additional Features
  • No Disposable Slides Required
  • Automated and Manual Size Gating
  • Autofocus
  • Dilution Calculator
  • Auto-Saved Data
  • LIMS Compatible Exporting

Example Data

AO/PI Hepatocyte Count

Automated Hepatocyte Counting: Live Demo

Want to see how the CellDrop achieves reliable automated hepatocyte counts? Watch the video below for an introduction to the live preview and count data available on the CellDrop Automated Cell Counter Hepatocytes app.

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Learn More: Additional Hepatocyte Resources

Count Cells Without Slides: DirectPipette™ Technology

The CellDrop Automated Cell Counter’s patented design replaces hemocytometers and plastic slides with an innovative wipe clean, variable height chamber.

1

Load Cell Suspension into Sample Chamber

2

Count, Analyze & Wipe Clean in Seconds

Wipe Clean 330 x 250
Features
  • Fast, reliable workflow
  • Use as little as 2.5 µL of sample
  • Two permanent, optical grade sapphire surfaces
  • Sample is held in place by surface tension
  • Three software-selectable chamber heights enable:
    • Widest dynamic range: 7 x 102 – 2.5 x 107 cells/mL
    • Cell size range: 4 – 400 µm

Slide Mode

Single-Use Plastic Slides: for hazardous samples or workflows that require sample containment

Reusable Slides: environmentally-friendly alternative to plastic slides when sample containment is required

Learn More about CellDrop Automated Cell Counters

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