This one is a game-changer! If you haven't used one yet, you are missing out on a great equipment for your otherwise tedious and inaccurate cell counting. The FL method is by far the best way to do this. I couldn't be happier!
Count Cells Without Slides
Reduce plastic waste, eliminate disposable slide costs, and automate cell counting in your lab with DeNovix CellDrop Automated Cell Counters.
Automated Cell Counter
Why Do Scientists Choose CellDrop?
DeNovix patented DirectPipette™ technology replaces hemocytometers and plastic slides traditionally used in cell counting equipment. The CellDrop is the only automated cell counter that eliminates the costs and plastic waste associated with cell counting slides.
Count Cells Without Slides
Automate counts, eliminate costs, and reduce plastic waste in your cell counting workflow.
Intuitive EasyApps™ Software
Easy-to-use, powerful software designed by life scientists, for life scientists.
Unmatched Performance
Fast, accurate and easy to use. Counts and viability in under 10 seconds.
Measurement Mode Flexibility
DirectPipette™ to eliminate plastic waste; reusable or single-use slides to suit your application.
Powerful Analysis & Connectivity
Wi-Fi, USB, Email, Ethernet and more. Data app automatically saves results.
Maintenance-Free Design
No recalibration required for the life of the instrument.
DirectPipette™ Mode
CellDrop’s patented DirectPipette™ technology replaces hemocytometers and plastic slides with an innovative wipe clean, variable height chamber.
1
Load Cell Suspension into Sample Chamber
2
Count, Analyze & Wipe Clean in Seconds
Features
- Fast, reliable workflow
- Use as little as 2.5 µL of sample
- Two permanent, optical grade sapphire surfaces
- Sample is held in place by surface tension
- Three software-selectable chamber heights enable:
- Widest dynamic range: 7 x 102 – 2.5 x 107 cells/mL
- Cell size range: 4 – 400 µm
Slide Mode
Single-Use Plastic Slides: for hazardous samples or workflows that require sample containment
Reusable Slides: environmentally-friendly alternative to plastic slides when sample containment is required
Reducing Plastic Waste in Cell Counting
The CellDrop’s innovative design improves efficiency and sustainability in cell counting equipment by removing the need for plastic slides. Scientists around the world have prevented millions of plastic slides from being disposed of by counting cells without slides.
Wide Variety of Applications
The CellDrop software excels in a wide variety of applications. View CellDrop technical notes to learn more.
Automated Brightfield and Fluorescence Cell Counters
Feature & Specification Comparison
Which CellDrop is right for your lab? Compare models with the full list of features and specifications.
 CellDrop FLi |  CellDrop BF |  CellDrop FLxi |  CellDrop BFx |
|
|---|---|---|---|---|
| Brightfield | ✅ | ✅ | ✅ | ✅ |
| Dual Fluorescence | ✅ | - | ✅ | - |
| Objective | 4X | 4X | 10X | 10X |
| Cell Size | 4 - 400 µm | 4 - 400 µm | 2 - 20 µm | 2 - 20 µm |
| Machine Learning Based Apps | ✅ | - | ✅ | - |
| Colors | Arctic White, Leaf Green | Arctic White, Leaf Green | Arctic White, Leaf Green | Arctic White, Leaf Green |
Powerful, Intuitive Software
Results
CellDrop results include a wide range of application-specific metrics for each cell sample. Data is clearly displayed on-screen and auto-saved to a large onboard hard drive. Metrics include:
Graphical Data Display
A wide range of graph types are available to gain additional sample insight. Overlay multiple samples from the same session or from the data archive for powerful data visualization.
Dilution Calculator
The convenient dilution calculator automatically calculates target dilution volumes of cell culture and buffer based on each cell count. Dilution guidance is displayed prominently on-screen and in printed reports for easy sample processing.
EasyApps™ Software
CellDrop EasyApps software is designed by life scientists, for life scientists. The system features a high definition, 7″ touchscreen and pre-installed apps for a range of common applications.
Additional apps for accounts, data, and other utilities contribute to the system’s easy-to-use interface.
Optimized Measurement Apps
DeNovix CellDrop Series instruments are stand-alone systems equipped with intuitive, easy-to-use apps for automatic cell counting and viability analysis.
Brightfield
- Count brightfield cell images in ~3 seconds
- Live/dead assessment using Trypan Blue or Erythrosin B
- Cells/mL, cell size, and % viability reported
- Nuclei concentration and extraction efficiency using Trypan Blue
Fluorescence
- Cell viability analysis using Acridine Orange (AO) and Propidium Iodide (PI) in ~8.5 seconds
- Analyze primary cells in the presence of debris
- Nuclei concentration and extraction efficiency using AO/PI assay
- Yeast viability
- GFP transfection efficiency
- Custom methods for other applications
Other Apps
Data App & Analysis
- All results and images are automatically saved and searchable in the Data App
- Large onboard memory enables storage and retrieval of thousands of results
- Analyze and optimize previous counts
- Easily export results and graphs
Exporting
Export results and images to USB, email, network folders, or printers. Export formats include PDF reports, .csv files, and .png files.
Counting Protocols
- Use built-in default protocols, or customize to streamline your workflow
- Create, save, and easily optimize protocols
- Custom settings include:
- Dilution factor
- Minimum / maximum diameter
- Cell / nuclei roundness
- Live / dead intensity threshold
- Sample mode (DirectPipette or Slide)
- Irregular cell mode
- Three chamber height options (400 µm, 100 µm, or 50 µm)
Protocol Settings Database
- CellDrop protocol settings for cells, primary cells, and nuclei
- Use as a starting point for your sample, or reach out to our applications team
Controls Toolbar
- Name sample
- Choose protocol
- Exposure
- Focus (includes Autofocus)
- Fluorescence channel selector
- Dilution calculator
Autofocus
Setting the focus on the CellDrop is easy. Because of the CellDrop’s fixed sample surfaces, adjusting focus is not often needed once it has been set. However, the software is also equipped with Autofocus. Just press the Auto button in the focus menu to automatically adjust to the correct focus.
Cell Size Gating
Cell / Nuclei Size Histograms allow easy gating for non-target cells or cell debris. Simply drag the slider to set the cell size of interest and view the results in real time. Results are instantly re-calculated excluding all debris or cells that fall outside of the defined size range
GFP Intensity Gating
When using the dedicated GFP App, users can gate cells by fluorescence intensity as well as size. The intensity histogram displays the number of cells counted at each GFP intensity level allowing the exclusion of non-GFP expressing cells or low expressing cells to be excluded from analysis.
Advanced Declustering Algorithms
All CellDrop Count Apps use proprietary declustering algorithms developed by DeNovix. Complex samples of regular or irregular cells over a wide range of cell density can be rapidly and accurately counted. Analysis of cluster size and frequency can be performed and graphically displayed.
Irregular Cell Mode
For cells that are elongated or irregularly shaped, enable Irregular Cell Mode from the Protocol Settings or the Optimize Setting button on the results screen. This is ideal for cells that are not round when trypsinized, such as fibroblasts or epithelial cells with a high degree of structure.
Irregular Cell Mode is also used to identify irregularly shaped isolated nuclei, such as smooth muscle nuclei from heart tissue.
IQ/OQ
A full installation and qualification (IQ/OQ) package is available for the CellDrop Automated Cell Counter.
Quote includes IQ/OQ documentation and reagents necessary for end-user completion of IQ/OQ process. On-site services are not provided by DeNovix.
Performance Verification Slide
The performance verification slide is a slide with beads fixed on the surface and is designed for assessing the accuracy and precision of a CellDrop Automated Cell Counter. Each slide includes a factory-certified value for the number of beads on the slide. Technical Note 224 provides instructions and calculation tables for validation.
A Splash of Color in Your Lab
CellDrop is available in two colors: Leaf Green and Arctic White.
21 CFR Part 11 Compliance Ready
EasyApps Secure provides an optional suite of software controls that allow regulated GxP facilities to easily add CellDrop to their cell counting workflow (view the compliance statement). The software is fully integrated within the onboard operating system and includes a range of features essential to ensuring compliance, including:
- Password protected system access
- Integrated electronic signature controls
- Comprehensive user account management
- Secure audit trail recording
- Advanced data handling and export tools
Hazardous Samples
The small footprint and onboard processing allow the CellDrop to fit inside most flow hoods. The instrument is also compatible with a range of disposable or reusable slides for hazardous samples that require containment.
Resources
Assays & Accessories
DeNovix CellDrop Assay Kits are simple to use and designed for Brightfield and Fluorescence applications preloaded on CellDrop instruments.
- Acridine Orange
- Propidium Iodide
- AO/PI Viability Assay
- Yeast
- Trypan Blue
- Erythrosin B
Reviews
The CellDrop has a 4.9 / 5 star rating on SelectScience with hundreds of independent customer reviews.
Coming from hand-counting cells, using the CellDrop has vastly improved my research by greatly saving me time. Plus, the user interface is very easy to use and learn. Getting to save counting protocols is great too. Overall, I would greatly recommend.
Austin Santhin
Using DeNovix CellDrop for almost a year I have had the best experience with it - easy-to-use interface, high quality camera, lots of built-in applications, almost no maintenance and of course no need to use cartridges or slides. Highly recommend to anyone looking for a good and reliable automatic cell counter.
Andrey Gorin
Simple to use and accurate, reduces waste and has a small footprint. After sales service is excellent.
Gillian Kay
Highly recommend for quick and accurate counts. Very pleased with this cell counter! Compact for benchtop and easy to use with no consumables needed. Excellent customer service and guidance whenever required.
Sara Silk
We were looking for a cell counter that would work for both cell culture and for analyzing fresh blood samples without too much dilution of the sample. We wanted something that was relatively fast and easy to use, reproducible and preferably didn’t produce much plastic waste. After having demoed five different instruments, CellDrop was the only one where we got it all, and it has additional features that can be very useful. Very happy with this piece of equipment!
Helena Fritz
CellDrop is so easy to use. I can count any type of cells in less then 30 seconds without the use of single use plastic. We save time, money and get more accurate counts, even with PBMCs.
Laurie Martineau
An excellent machine that's quite straightforward to use while having a surprising number of features. It's easy to use and easy to clean. Can do brightfield, trypan blue, fluorescence, etc.
Allison Cote
Purchase Options
Unlimited
- Unlimited number of measurements can be made at no additional cost
- Ideal for complete flexibility and freedom in counting
Pay-As-You-Go
- Lower initial investment
- Purchase Count Codes as needed for a specific number of measurements
- Ideal solution for labs with smaller capital budgets / lower sample throughput looking to acquire high performance
- Can be upgraded to Unlimited at any time
- 50% of the cost of purchased codes goes towards upgrading to CellDrop Unlimited
In this eBook, you’ll find resources that discuss methods for improved cell counting accuracy, including a comparison of trypan blue and AO/PI fluorescence viability dyes.
The CellDrop Automated Cell Counter counts cells without slides, which reduces plastic waste in science labs. But how many plastic slides has it actually saved, and how much plastic is that? Watch this video to find out!
Technical Note 231 highlights an application note from Miltenyi Biotec on optimizing nuclei preparation workflow for genomic applications.
