AO/PI Viability Assay
The Acridine Orange (AO) / Propidium Iodide (PI) assay is used for cell counting and viability. AO is a nucleic acid-binding fluorophore that is cell membrane permeable and suitable for selective staining of nucleated cells. PI is a nucleic acid-binding dye that is impermeable to live cells and suitable for staining dead, nucleated cells. All live, nucleated cells fluoresce green due to AO, while dead, nucleated cells are stained with both AO and PI and fluoresce red. Available in 300 assay or 1500 assay quantities.
SKU: K-30520
Components – 1.5 mL (300 assays) @ mix of 5 ug/mL AO+ 100 ug/mL PI in PBS
SKU: K-30530
Components – 7.5 mL (1500 assays) @ mix of 5 ug/mL AO+ 100 ug/mL PI in PBS
Acridine Orange
The Acridine Orange (AO) Dye is used for total cell counting. AO is a nucleic acid-binding fluorophore that is cell membrane permeable and suitable for selective staining of nucleated cells.
SKU: K-30500
Components 1.5 mL @ 10 ug/mL in PBS
300 Assays
Propidium Iodide
The Propidium Iodide (PI) Dye is used for viability assessment. PI is a nucleic acid-binding dye that is impermeable to live cells and suitable for staining dead, nucleated cells.
SKU: K-30570
Components 1.5 mL @ 100 ug/mL in PBS
300 Assays
Yeast
The Fluorescein Diacetate (FDA) and Propidium Iodide (PI) assay is used for yeast cell viability testing. FDA is a cell viability probe that is membrane-permeable to live yeast cells. Inside a live cell, FDA is cleaved to give fluorescein by the action of intracellular esterases. Propidium Iodide dye for viability assessment. PI is a nucleic acid-binding dye that is impermeable to live cells and suitable for staining dead, nucleated cells. Live cells are reported by FDA in the green channel, dead cells by PI in the red channel.
SKU: K-30590
Components 300 uL PI, 300 uL FDA, 25 mL PBS buffer
300 Assays
Trypan Blue
The Trypan Blue (TB) dye is used for brightfield viability assessment. TB is an azo dye that is excluded from cells with intact cell membranes, only staining non-viable cells. The trypan blue assay is used to assess the number of viable cells present in a cell suspension.
SKU: K-30580
Components 1.5 mL @ 0.4% in PBS
300 Assays
Erythrosin B
Erythrosin B is a biosafe and non-toxic colorimetric dye that can be used as an alternative to Trypan Blue for cell counting and viability assessment. Evaluation of cell viability using Erythrosin B dye exclusion is based on a similar principle to Trypan Blue, that intact cell membranes exclude the polar molecules whereas compromised membranes do not. Live cells remain unstained while dead cells are stained and have a darker appearance.
SKU: K-30490
300 Assays
Count Codes for CellDrop Cell Counters
The Serial Number is required. Please include when placing your order.
The DeNovix team will process the order and email a count code within one business day. If you have an emergency requirement for additional counts, please call DeNovix at +1-302-442-6911 for immediate help, 9:00 am – 5:00 pm Eastern US time.
Brightfield Beads for CellDrop
SKU: K-30540
Components 0.5 mL @ 1×106
50 Samples
Fluorescence Beads for CellDrop (Fluorescence Models)
SKU: K-30550
Components 0.5 mL green / red mix @ 1×106
50 Samples
Accessories
DeNovix 20 µL Pipette
20 uL Pipette
Capacity 2.0 – 20.0 uL
Ergonomic design
Performance Verification Slide
SKU: P-0528
The CellDrop PV Slide is used to confirm CellDrop accuracy and precision. The slide contains permanently mounted beads which are analyzed and labeled with a factory certified count and density. The slide can be used for validation of the brightfield, green fluorescence and red fluorescence channels. Factory validation certificate and storage case are included.
Opticon USB Scanner OPI3601
> Rapidly scans/decodes a wide variety of 1D or 2D barcodes
> Customizable trigger modes
> Distinct targeting guides
> Auto-Trigger features both stand-alone and with stand detection