Dr. Shawn Mehlenbacher is part of the Hazelnut Breeding and Genetics program at Oregon State University.
In a paper recently published in Tree Genetics & Genomes (2021) 17: 6, Hill et al. describe fine mapping of the locus that controls pollen-stigma incompatibility in hazelnut, including development of new simple sequence repeat and single nucleotide polymorphism markers. The S1 haplotype is from the resistant parent OSU 414.062 and the S3 haplotype is from the susceptible parent OSU 252.146.
The team used the DeNovix QFX Fluorometer in conjunction with the DeNovix dsDNA High Sensitivity Assay to quantify their extracted dsDNA prior to sequencing the parent genomes. These sequencing data were then used to develop polymorphic DNA markers and predict genes in the aligned sequences.
