Tag Archives: Cell Viability

A detailed description of how to optimize the AOPI or Custom Methods Applications for counting plant protoplasts on the DeNovix CellDrop™ FL Automated Cell Counter in conjunction with fluorescein diacetate (FDA) and propidium iodide(PI) fluorescent dyes.

Many yeast samples from brewing contain extracellular debris and are small in size, making them difficult to count using a traditional hemocytometer or brightfield cell counter. This technical note will provide the information needed to accurately count yeast concentration and viability using the fluorescent dyes fluorescein diacetate (FDA) and propidium iodide (PI).

The use of trypan blue can be problematic for counting cells and assessing viability in some sample types where debris or non-nucleated cells can interfere. The DeNovix Acridine Orange (Ao) / Propidium Iodide (PI) fluorescence assay is a simple, accurate alternative. Comparative data is presented here.

The NIST has proposed a robust method to assess cell counting linearity and reproducibility. This technical note presents data for the CellDrop using this method and compares results to manually cell counting.