This blog post from DeNovix explores the four primary protein quantification assays used in microvolume analysis of proteins and biochemistry applications.
The choice of measurement method for proteins depends on sample type, buffers used and the protein of interest. Colorimetric assays are commonly used for cell lysates and samples in buffers that interfere with direct quantification at 280nm. Common assays and results using microvolume analysis are presented in technical note 108.
Microvolume spectrophotometric measurements are now routine in many life science research labs. As with all microvolume techniques, it is important to apply the best practices to reduce user error and ensure reliable results from a DeNovix DS-11 Series instrument.
