In this post, DeNovix explores primary cell counting using AO/PI with a focus on peripheral blood mononuclear cells (PBMCs).
Fluorometric sample quantification is a routine application in molecular biology labs however it is important to employ a number of best practices to ensure reliable results. This document outlines some of the key considerations.
Fluorometric sample quantification is a routine application in molecular biology labs however it is important to employ a number of best practices to ensure reliable results. This document outlines some of the key considerations.
Viability is a key metric in determining the activity of yeast cells in solution using fluorescent stains like FDA and PI with both cell counters.
Despite the variety of cell counters available, they can typically be divided into one of two sub-groups: manual and automated cell counters.
The DeNovix dsDNA High Sensitivity Assay dye selectively binds dsDNA over RNA, ssDNA or protein. This assay is used to measure samples with concentrations ranging between 5 pg/µL – 250 ng/µL. Performance data is presented using the DS-11 FX.
The DeNovix dsDNA Ultra High Sensitivity Assay dye selectively binds dsDNA over RNA, ssDNA or protein. This assay is used to measure samples with very low concentrations, between 0.5 and 300 pg/µL.
This detailed technical note describes the DeNovix® dsDNA Ultra High Sensitivity Assay, which enables the accurate detection of purified double-stranded DNA (dsDNA) samples with a detection range from 5 pg to 3 ng total mass per assay tube.
The DeNovix dsDNA Ultra High Sensitivity Assay enables the accurate detection of purified double-stranded DNA (dsDNA) samples with a detection range from 5 pg to 3 ng total mass per assay tube.
This detailed technical note describes the DeNovix® dsDNA High Sensitivity Assay, which enables accurate detection of purified double-stranded DNA (dsDNA) samples with a standard detection range from 100 pg to 250 ng total mass in 200 µL volumes.
