The CellDrop FLxi and AO/PI fluorescence viability stain combine to provide reliable yeast cell counts and viability for the brewing industry.
The Yeast app on CellDrop Series instruments enables rapid automated cell counting for live/dead yeast cell suspensions.
Many yeast samples from brewing contain extracellular debris and are small in size, making them difficult to count using a traditional hemocytometer or brightfield cell counter. This technical note will provide the information needed to accurately count yeast concentration and viability using the fluorescent dyes fluorescein diacetate (FDA) and propidium iodide (PI).
Viability is a key metric in determining the activity of yeast cells in solution using fluorescent stains like FDA and PI with both cell counters.
