The Power of Fluorescence for Quantification of Nucleic Acid Samples: Expert Tips and Tricks

Absorbance and fluorescence analysis are distinct yet complementary techniques used to detect and quantify analytes of interest in a sample. Measuring the absorbance of ultraviolet and visible (UV-Vis) light at specific wavelengths is one of the most established methods of quantitating biomolecules. Fluorescence analysis, meanwhile, has long been used to measure the emission spectra of fluorophores binding to samples excited by wavelengths for highly specific molecular quantification.

During this presentation, we will cover

1 – Absorbance vs Fluorescence: Sensitivity, specificity, ease of use and cost. Which method is best for your application?
2 – Best Practices: Common sources of error and how to avoid them.
3- Hardware: what’s needed for regular lab use and higher throughput?